Buy Bromophenol Blue from ReAgent UK
With over 40 years of experience, ReAgent is internationally recognised for its high-quality products and industry-leading customer service.
ReAgent supply powdered bromophenol blue in 5 kg and 25 kg containers. We can also supply ready-made 0.04% bromophenol solution in 500 ml and 2.5 L containers. Buy from our online chemicals store where you can get discounts on bulk orders and nationwide next-day delivery.
Bromophenol blue can be used as a pH indicator, a dye and a colour marker. It’s usually prepared by gently mixing bromine with phenolsulfonphthalein in glacial acetic acid.
- pH indicator: it changes colour from yellow at pH 3 to purple at pH 4.6 in a reversible reaction. In chemical terms, the reaction is akin to what happens to phenolphthalein, another popular pH indicator.
- Colour marker: it is popularly used to monitor progress during electrophoresis (both agarose gel and polyacrylamide gel). As this compound has a slightly negative charge, it will travel in the gel closely to the DNA or protein being tested. For example, using a 1% agarose gel in TAE or TBE buffer, bromophenol blue travels at the same speed as a 500 base pair DNA fragment.
- Dye: At pH 7 and above, this compound absorbs red light and transmits blue light, which means solutions are blue. In acidic solutions (pH<7), this dye absorbs ultraviolet light and transmits in the yellow range. Interestingly, at pH 3.6 (when the colour changes), this compound has a green or red colour depending on the concentration. This is called dichromatic colour.
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